diff --git a/BDAccess/app.R b/BDAccess/app.R
index 1c89fba..e1b54fe 100644
--- a/BDAccess/app.R
+++ b/BDAccess/app.R
@@ -1169,6 +1169,11 @@ server <- function(input, output) {
                           # plot.margin = margin(-200,0,0,0),
                           axis.text = element_blank())
                 
+                nodes<-gs_get_pop_paths(gs)
+                nodes_parent<-nodes[!grepl("CTLA4|LAG3|PD1|TIGIT|TIM3|root$", nodes)]
+                nodes_cd4<-nodes[grepl("CTLA4$|LAG3$|PD1$|TIGIT$|TIM3$", nodes) & grepl("/CD4/",nodes)]
+                nodes_cd8<-nodes[grepl("CTLA4$|LAG3$|PD1$|TIGIT$|TIM3$", nodes) & grepl("/CD8/",nodes)]
+                
                 # g1<-ggcyto_arrange(autoplot(gs[[ab]], nodes_parent, bins=128), nrow=1)
                 # g2<-ggcyto_arrange(autoplot(gs[[iso]], nodes_cd8, bins=64), nrow=1)
                 # g3<-ggcyto_arrange(autoplot(gs[[ab]], nodes_cd8, bins=64), nrow=1)