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					@ -45,7 +45,26 @@ ui <- fluidPage( | 
				
			
			
		
	
		
			
				
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					                         h3('Estadística'), | 
				
			
			
		
	
		
			
				
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					                         verbatimTextOutput('stats'), | 
				
			
			
		
	
		
			
				
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					                         tableOutput('tab_stats') | 
				
			
			
		
	
		
			
				
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					                       )) | 
				
			
			
		
	
		
			
				
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					                       )), | 
				
			
			
		
	
		
			
				
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					              tabPanel("Exportar", | 
				
			
			
		
	
		
			
				
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					                       sidebarPanel(width=2, | 
				
			
			
		
	
		
			
				
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					                                    h3('Seleccionar figura'), | 
				
			
			
		
	
		
			
				
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					                                    selectInput("fig_id", "", selected="", choices=c("Cinética Grupo", "Cinética Individual", "Survival")), | 
				
			
			
		
	
		
			
				
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					                                    h3('Formato'), | 
				
			
			
		
	
		
			
				
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					                                    sliderInput("width", "Ancho", min=1000, max=20000, step=1000, value=10000), | 
				
			
			
		
	
		
			
				
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					                                    sliderInput("height", "Altura", min=1000, max=20000, step=1000, value=6000), | 
				
			
			
		
	
		
			
				
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					                                    textInput("colors", label="Colors", value=""), | 
				
			
			
		
	
		
			
				
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					                                    sliderInput("errorbar-width", "% Ancho errorbars", min=0.05, max=1, step=0.05, value=0.05), | 
				
			
			
		
	
		
			
				
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					                                    sliderInput("point-size", "Tamaño puntos", min=1, max=10, step=1, value=3), | 
				
			
			
		
	
		
			
				
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					                                    sliderInput("font-size", "Tamaño textos", min=5, max=30, step=1, value=11), | 
				
			
			
		
	
		
			
				
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					                                    checkboxInput(inputId = "legend", label = "Mostrar llegenda", value = T), | 
				
			
			
		
	
		
			
				
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					                                    selectInput("theme", "Seleccionar Tema", selected="BW", choices=c("BW", "Default", "Classic")), | 
				
			
			
		
	
		
			
				
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					                                    downloadButton("downloadPicture", "Exportar") | 
				
			
			
		
	
		
			
				
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					                       ), | 
				
			
			
		
	
		
			
				
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					                       mainPanel( | 
				
			
			
		
	
		
			
				
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					                         uiOutput("expPlotUI") | 
				
			
			
		
	
		
			
				
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					                       ) | 
				
			
			
		
	
		
			
				
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					              ) | 
				
			
			
		
	
		
			
				
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					              ) | 
				
			
			
		
	
		
			
				
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					    | 
				
			
			
		
	
		
			
				
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					   ) | 
				
			
			
		
	
	
		
			
				
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					@ -229,9 +248,12 @@ server <- function(input, output) { | 
				
			
			
		
	
		
			
				
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					      if ("ID.tumor" %in% colnames(table)){table<-rename(table, "ID tumor"=`ID.tumor`)} | 
				
			
			
		
	
		
			
				
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					      # table[table$ID.tumor == "R","0"]<-NA | 
				
			
			
		
	
		
			
				
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					      col_nodays<-c("ID", "Cage","Group", "ID.animal","ID animal", "ID.tumor", "ID tumor", "TS","DPV", "Absorbance") | 
				
			
			
		
	
		
			
				
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					      print(grep(0, colnames(table)[!colnames(table) %in% col_nodays])) | 
				
			
			
		
	
		
			
				
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					      if (length(grep(0, colnames(table)[!colnames(table) %in% col_nodays])) == 0){ | 
				
			
			
		
	
		
			
				
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					        table["0"]<-0 | 
				
			
			
		
	
		
			
				
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					      } | 
				
			
			
		
	
		
			
				
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					      table<-melt(table, id=colnames(table)[colnames(table) %in% col_nodays], variable.name = "Timepoint") | 
				
			
			
		
	
		
			
				
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					      table$Timepoint<-gsub("[A-Za-z ]","",table$Timepoint) | 
				
			
			
		
	
		
			
				
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					      table$Timepoint<-factor(table$Timepoint, levels=mixedsort(as.numeric(as.character(unique(table$Timepoint))))) | 
				
			
			
		
	
		
			
				
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					      if ("DPV" %in% colnames(table)){ | 
				
			
			
		
	
		
			
				
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					        table<-dcast(table, Cage+`ID animal`+`ID tumor`+Group+Timepoint~DPV, value.var = "value") | 
				
			
			
		
	
		
			
				
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					        table$Major<-table$Major/1000 | 
				
			
			
		
	
	
		
			
				
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					@ -244,6 +266,7 @@ server <- function(input, output) { | 
				
			
			
		
	
		
			
				
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					      } | 
				
			
			
		
	
		
			
				
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					      if (!"Volume" %in% colnames(table)){table<-rename(table, "Volume"=value)} | 
				
			
			
		
	
		
			
				
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					      table<-table %>% filter(!is.na(Group)) | 
				
			
			
		
	
		
			
				
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					      table$Timepoint<-factor(table$Timepoint, levels=mixedsort(as.numeric(as.character(unique(table$Timepoint))))) | 
				
			
			
		
	
		
			
				
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					      analysis$taula_def<-table | 
				
			
			
		
	
		
			
				
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					      table_plot<-dcast(dcast(table %>% filter(!is.na(Volume)), `ID animal`+Group+Timepoint~., value.var = "Volume", fun.aggregate = mean), Group~Timepoint) | 
				
			
			
		
	
		
			
				
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					      table_plot | 
				
			
			
		
	
	
		
			
				
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					@ -373,6 +396,88 @@ server <- function(input, output) { | 
				
			
			
		
	
		
			
				
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					      } | 
				
			
			
		
	
		
			
				
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					    } | 
				
			
			
		
	
		
			
				
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					  }) | 
				
			
			
		
	
		
			
				
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					  output$expPlotUI<- renderUI({ | 
				
			
			
		
	
		
			
				
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					    observeEvent(analysis$taula_def, {}) | 
				
			
			
		
	
		
			
				
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					    if (!is.null(input$file_analy) & !is.null(analysis$taula_def)){ | 
				
			
			
		
	
		
			
				
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					      plotOutput("expPlot", width=paste0(input$width/10,"px"), height = paste0(input$height/10, "px")) | 
				
			
			
		
	
		
			
				
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					    } | 
				
			
			
		
	
		
			
				
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					  }) | 
				
			
			
		
	
		
			
				
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					  output$expPlot <- renderPlot({ | 
				
			
			
		
	
		
			
				
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					    observeEvent(analysis$taula_def, {}) | 
				
			
			
		
	
		
			
				
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					    if (!is.null(input$file_analy) & !is.null(analysis$taula_def)){ | 
				
			
			
		
	
		
			
				
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					      table<-analysis$taula_def | 
				
			
			
		
	
		
			
				
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					      print(head(table)) | 
				
			
			
		
	
		
			
				
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					      if (input$fig_id == "Cinética Grupo"){ | 
				
			
			
		
	
		
			
				
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					        if (input$vacc == "Sí"){ | 
				
			
			
		
	
		
			
				
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					          g<-ggplot(table, aes(as.numeric(as.character(Timepoint)), Volume, color=Group, group=Group))+ | 
				
			
			
		
	
		
			
				
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					            scale_x_continuous(expand = expansion(mult = c(0,0.05)), limits = c(0, (round(max(as.numeric(as.character(table$Timepoint))) / 5)+1)*5))+ | 
				
			
			
		
	
		
			
				
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					            facet_grid(factor(`ID tumor`, labels = c("Vaccination", "Rechallenge"))~., scale="free_y")+ | 
				
			
			
		
	
		
			
				
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					            theme_bw()   | 
				
			
			
		
	
		
			
				
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					        }else{ | 
				
			
			
		
	
		
			
				
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					          g<-ggplot(table, aes(Timepoint, Volume, color=Group, group=Group))+ | 
				
			
			
		
	
		
			
				
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					            theme_bw() | 
				
			
			
		
	
		
			
				
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					        } | 
				
			
			
		
	
		
			
				
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					      } | 
				
			
			
		
	
		
			
				
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					      if (input$fig_id == "Cinética Individual"){ | 
				
			
			
		
	
		
			
				
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					        if (input$vacc == "Sí"){ | 
				
			
			
		
	
		
			
				
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					          g<-ggplot(table, aes(as.numeric(as.character(Timepoint)), Volume, color=Group, group=Group))+ | 
				
			
			
		
	
		
			
				
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					            scale_x_continuous(expand = expansion(mult = c(0,0.05)), limits = c(0, (round(max(as.numeric(as.character(table$Timepoint))) / 5)+1)*5))+ | 
				
			
			
		
	
		
			
				
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					            facet_grid(factor(`ID tumor`, labels = c("Vaccination", "Rechallenge"))~Group, scale="free_y")+ | 
				
			
			
		
	
		
			
				
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					            theme_bw()   | 
				
			
			
		
	
		
			
				
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					        }else{ | 
				
			
			
		
	
		
			
				
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					          g<-ggplot(table, aes(Timepoint, Volume, color=Group, group=`ID animal`))+ | 
				
			
			
		
	
		
			
				
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					            facet_wrap(.~Group)+ | 
				
			
			
		
	
		
			
				
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					            theme_bw() | 
				
			
			
		
	
		
			
				
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					        } | 
				
			
			
		
	
		
			
				
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					      } | 
				
			
			
		
	
		
			
				
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					      g<-g+geom_errorbar(stat="summary", width=input$`errorbar-width`)+ | 
				
			
			
		
	
		
			
				
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					        geom_line(stat="summary")+ | 
				
			
			
		
	
		
			
				
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					        geom_point(stat="summary", size=input$`point-size`)+ | 
				
			
			
		
	
		
			
				
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					        labs(x="Days after tumor challenge")+ | 
				
			
			
		
	
		
			
				
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					        scale_y_continuous(expand = expansion(mult = c(0,0.05))) | 
				
			
			
		
	
		
			
				
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					      if (input$theme == "BW"){ | 
				
			
			
		
	
		
			
				
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					        g<-g+theme_bw(base_size = input$`font-size`) | 
				
			
			
		
	
		
			
				
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					      } | 
				
			
			
		
	
		
			
				
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					      if (input$theme == "Classic"){ | 
				
			
			
		
	
		
			
				
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					        g<-g+theme_classic(base_size = input$`font-size`)   | 
				
			
			
		
	
		
			
				
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					      } | 
				
			
			
		
	
		
			
				
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					      if (input$theme == "Default"){ | 
				
			
			
		
	
		
			
				
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					        g<-g+theme_gray(base_size = input$`font-size`) | 
				
			
			
		
	
		
			
				
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					      } | 
				
			
			
		
	
		
			
				
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					      # g<-g+theme(axis.text.x=element_text(angle=45, hjust=1)) | 
				
			
			
		
	
		
			
				
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					      if (input$legend == F){ | 
				
			
			
		
	
		
			
				
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					        g<-g+guides(color=FALSE, fill=FALSE) | 
				
			
			
		
	
		
			
				
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					      } | 
				
			
			
		
	
		
			
				
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					      if (input$colors != ""){ | 
				
			
			
		
	
		
			
				
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					        v_col<-strsplit(input$colors, ",")[[1]] | 
				
			
			
		
	
		
			
				
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					        g<-g+scale_color_manual(values=v_col)+ | 
				
			
			
		
	
		
			
				
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					          scale_fill_manual(values=v_col) | 
				
			
			
		
	
		
			
				
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					      } | 
				
			
			
		
	
		
			
				
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					      dades$plot<<-g | 
				
			
			
		
	
		
			
				
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					      g | 
				
			
			
		
	
		
			
				
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					    } | 
				
			
			
		
	
		
			
				
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					  }, res=72) | 
				
			
			
		
	
		
			
				
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					  output$downloadPicture <- downloadHandler( | 
				
			
			
		
	
		
			
				
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					    filename = function() { | 
				
			
			
		
	
		
			
				
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					      paste("Figura", ".png", sep="") | 
				
			
			
		
	
		
			
				
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					    }, | 
				
			
			
		
	
		
			
				
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					    content = function(file){ | 
				
			
			
		
	
		
			
				
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					      print(file) | 
				
			
			
		
	
		
			
				
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					      # tempReport <- file.path(tempdir(), "elispots.Rmd") | 
				
			
			
		
	
		
			
				
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					      # file.copy("elispots.Rmd", tempReport, overwrite = TRUE) | 
				
			
			
		
	
		
			
				
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					      png(file, width = input$width, height=input$height, units = "px", res=720) | 
				
			
			
		
	
		
			
				
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					      plot(dades$plot) | 
				
			
			
		
	
		
			
				
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					      dev.off() | 
				
			
			
		
	
		
			
				
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					    }) | 
				
			
			
		
	
		
			
				
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					} | 
				
			
			
		
	
		
			
				
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					# Run the application  |