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| # scMonitor | |||
| scMonitor is a shinny application writen in R that allows to easily explore already processed scRNAseq seurat objects. | |||
| Here, there is a video showing its features: | |||
| <video width="720" height="420" controls> | |||
| <source src="scMonitor.webm" type="video/webm"> | |||
| <track src="scMonitor.eng.vtt" kind="subtitles" srclang="en" label="English" default> | |||
| <track src="scMonitor.es.vtt" kind="subtitles" srclang="es" label="Spanish"> | |||
| </video> | |||
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| WEBVTT | |||
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| In this video, I will present you | |||
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| the shinny application scMonitor, which is | |||
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| oriented to easily explore | |||
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| a previously processed seurat object. | |||
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| Firstly, you should select the file | |||
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| containing the seurat object. | |||
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| For now, the file format must be RDS. | |||
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| Depending on the object size and | |||
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| computer potency the loading | |||
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| will take more or less time. | |||
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| UMAP distribution that generates | |||
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| is the same that shows as default, | |||
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| usually clusters identified by seurat itself. | |||
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| We can group colors by any | |||
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| other variable that we have included in the | |||
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| metadata from the object specifying it in "GroupBy". | |||
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| Tipically, the sample origin or | |||
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| a manual annotation | |||
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| from the cell type. | |||
| 00:48.800 --> 00:51.400 | |||
| Distribution can also be divided by | |||
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| another variable selecting in "FacetBy". | |||
| 00:54.500 --> 00:57.300 | |||
| In this case, we are looking at | |||
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| the distribution of cell type | |||
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| by the sample origin. | |||
| 01:02.800 --> 01:05.800 | |||
| Another available visualization is to show expression | |||
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| for a group of genes separated by a white space | |||
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| over UMAP distribution. | |||
| 01:11.300 --> 01:13.500 | |||
| With the "Height" bar we can adjust | |||
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| the size in order to feel comfortable. | |||
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| Finally, we can create signatures in order to visualize | |||
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| their score from cells. | |||
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| One must indicate the signature name and | |||
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| on the box below specify the genes | |||
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| that are included. Once added, you can | |||
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| specify its name in the | |||
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| signatures box. | |||
| @ -0,0 +1,109 @@ | |||
| WEBVTT | |||
| 00:00.700 --> 00:02.600 | |||
| En este vídeo os voy a presentar | |||
| 00:02.600 --> 00:05.500 | |||
| la aplicación "shinny" scMonitor, que está | |||
| 00:05.500 --> 00:08.200 | |||
| orientada a explorar de forma sencilla un | |||
| 00:08.200 --> 00:10.300 | |||
| objeto seurat previamente procesado. | |||
| 00:11.600 --> 00:13.800 | |||
| Lo primero es seleccionar el fichero que | |||
| 00:13.800 --> 00:16.800 | |||
| contiene ese objeto seurat. Por ahora, | |||
| 00:16.800 --> 00:19.000 | |||
| el formato del fichero debe RDS. | |||
| 00:19.700 --> 00:21.600 | |||
| En función del tamaño del objeto y | |||
| 00:21.600 --> 00:23.800 | |||
| de la potencia del ordenador la carga | |||
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| tardará más o menos. | |||
| 00:26.200 --> 00:28.200 | |||
| La distribución UMAP que se genera | |||
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| es la que se muestra por defecto, | |||
| 00:30.400 --> 00:33.400 | |||
| normalmente los "clusters" identificados por el propio seurat. | |||
| 00:33.400 --> 00:37.200 | |||
| Podemos agrupar los colores por cualquier | |||
| 00:37.200 --> 00:39.300 | |||
| otra variable que hayamos incluido en los | |||
| 00:39.300 --> 00:42.600 | |||
| metadatos del objeto especificándolo en "GroupBy". | |||
| 00:43.200 --> 00:45.500 | |||
| Típicamente, el origen de la muestra o | |||
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| una anotación | |||
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| manual de tipo celular. | |||
| 00:48.800 --> 00:51.400 | |||
| También se puede dividir la distribución en | |||
| 00:51.400 --> 00:54.500 | |||
| función de una variable seleccionándolo en "FacetBy". | |||
| 00:54.500 --> 00:57.300 | |||
| En este caso estamos viendo la | |||
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| distribución de los tipos celulares en función | |||
| 00:59.900 --> 01:01.000 | |||
| del origen de la muestra. | |||
| 01:02.800 --> 01:05.800 | |||
| Otra visualización disponible es mostrar la expresión | |||
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| de conjuntos de genes separados por un | |||
| 01:08.100 --> 01:10.400 | |||
| espacio sobre la distribución UMAP. | |||
| 01:11.300 --> 01:13.500 | |||
| Con la barra de altura podemos ajustar | |||
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| el tamaño para que nos sea cómodo. | |||
| 01:18.900 --> 01:22.700 | |||
| Finalmente, podemos crear signaturas para luego visualizar | |||
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| el "score" de las células. | |||
| 01:25.200 --> 01:27.300 | |||
| Hay que indicar el nombre de la signatura y | |||
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| en la casilla inferior especificar los genes | |||
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| que incluye. Una vez añadida se puede | |||
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| especificar su nombre en la casilla de | |||
| 01:34.700 --> 01:35.500 | |||
| signaturas. | |||